The process starts with the collection of both patient blood and a tumor biopsy.

Whole-Genome Sequencing data from matched tumor and normal DNA is required for each patient.

Following alignment of these reads to the genome, somatic gene alterations in the tumor genome are detected using a collection of algorithms called 'neoX'.

Next, these somatic gene alterations are translated in-silico to obtain the tumor-specific peptides

In a last step, the peptides are prioritized using an immunogenicity predictor (neoIM) and two presentation predictors (neoMS and MHCrank).